Homing Endonuclease I-CreII: A Novel Dual-Motif Enzyme That Catalyzes Group I Intron Homing
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چکیده
Acknowledgements I would like to thank the past and present members of the Herrin lab for an excellent graduate school experience. Special thanks go to Dr. O.W. Odom for his help and guidance with experimental design, troubleshooting, and data analysis. I am also indebted to Kristina Mahan and Weihua Quo for critical reading of this manuscript.
منابع مشابه
Biochemical and mutagenic analysis of I-CreII reveals distinct but important roles for both the H-N-H and GIY-YIG motifs
Homing endonucleases typically contain one of four conserved catalytic motifs, and other elements that confer tight DNA binding. I-CreII, which catalyzes homing of the Cr.psbA4 intron, is unusual in containing two potential catalytic motifs, H-N-H and GIY-YIG. Previously, we showed that cleavage by I-CreII leaves ends (2-nt 3' overhangs) that are characteristic of GIY-YIG endonucleases, yet it ...
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